RNA cleanup spin columns for varying input amounts—NucleoSpin RNA Clean-up
The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g, amplification or labeling reactions), DNase digestions, and more. The kits enable complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the protocol employed by the NucleoSpin RNA purification kits, excluding the DNase digestion and homogenization steps.
The NucleoSpin RNA Clean-up kits provide a simple, fast, and convenient way to clean up your RNA from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g, amplification or labeling reactions), DNase digestions, and more. The kits enable complete removal of RT-PCR inhibitors, leading to superior RT-PCR results. The time-saving procedure is based on the protocol employed by the NucleoSpin RNA purification kits, excluding the DNase digestion and homogenization steps.
NucleoSpin RNA Clean-up is also recommended for RNA isolation from cultured cells whenever copurification of some genomic DNA is acceptable. For isolating RNA from cells and tissue with the least DNA contamination, we recommend the rDNase-containing NucleoSpin RNA purification kits. These kits enable preparation of pure RNA with an A260/280 ratio generally exceeding 1.9 (measured in TE buffer, pH 7.5).
RNA purified with the NucleoSpin RNA Clean-up kits is ready to use in applications such as enzymatic labeling reactions (including dye incorporation), reverse transcriptase-PCR (RT-PCR), and DNA/RNA-based chip hybridizations. The NucleoSpin RNA Clean-up kits can also be used to clean up in vitro-transcribed RNA and aminoallyl mRNA, as well as biotinylated and fluorescently labeled RNA.
Overview
- Complete removal of RT-PCR inhibitors
- Time-saving procedure based on NucleoSpin RNA, without DNase digestion and homogenization steps
- RNA cleanup from prepurified RNA (phenol/chloroform), enzymatic reactions (e.g., amplification reactions, labeling reactions, or DNase digestions)
- Competitive price per prep
More Information
| NucleoSpin RNA Clean-up |
NucleoSpin RNA Clean-up XS | NucleoSpin RNA Clean-up Maxi | |||||
| Technology | Silica membrane | ||||||
| Format | Mini spin columns | Mini spin column (XS design) |
Maxi spin column | ||||
| Starting material |
<200 µl RNA sample |
<300 µl RNA sample containing <90 µg RNA | <7.5 ml RNA sample containing <35 mg RNA | ||||
| Fragment size | >200 nt | ||||||
| Typical recovery | 85–95% | ||||||
| A260/280 | 1.9–2.1 | ||||||
| Elution volume | 40–120 µl | 5–30 µl | 3–5 ml | ||||
| Preparation time | 20 min/6 preps | 20 min/6 preps | 30 min/6 preps | ||||
| Binding capacity | 200 µg | 110 µg | 35 mg | ||||
Applications
Clean up the following samples:
- Prepurified RNA (e.g., Trizol)
- Reaction mixtures
- Aminoallyl mRNA
- Biotinylated RNA
- RNA isolation from up to 105 cultured cells (whenever copurification of some genomic DNA is acceptable, since the kit does not contain rDNase)
Obtain RNA ready to use for:
- Enzymatic labeling reactions
- RT-PCR
- DNA/RNA-based chip hybridizations
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
The better way to isolate RNA
Critical factors when purifying RNA are the complete removal of contaminating DNA (gDNA) and the immediate prevention of degradation of RNA. The NucleoSpin RNA Plus kit introduces the NucleoSpin gDNA Removal Column, a spin column which quickly and completely removes genomic DNA contamination without the need for DNase digestion. To maintain RNA integrity, cells and tissues are first lysed by incubation in a chaotropic ion lysis buffer solution, which immediately inactivates RNases.
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