Isolation of genomic DNA from stool samples—NucleoSpin DNA Stool
NucleoSpin DNA Stool is designed for purification of genomic DNA from bacterial and epithelial cells present in stool samples. The isolated DNA is used for analysis of the microbiome or genotyping of the donor. The kit utilizes MN Bead Tubes Type A (ceramic beads; included in the kit) in combination with a common disruption device, e.g., an MN Bead Tube Holder or a swing mill, to lyse different cell types and tissues using a single procedure. NucleoSpin Inhibitor Removal Columns are included for convenient elimination of contaminants.
Overview
- Suitable for any stool sample—high-quality DNA from stool from carnivores, omnivores, and herbivores
- Includes MN bead tubes for efficient lysis—compatible with the most common disruption devices
- NucleoSpin Inhibitor Removal Columns ensure superior DNA purity
More Information
| Technology | Silica-membrane technology combined with MN Bead Tubes Type A |
| Format | Mini spin columns |
| Starting material | Approximately 200 mg (180–220 mg)* stool samples (fresh or frozen) |
| Fragment size | 200 bp–approximately 50 kb |
| Typical yield | Depends on sample type, quality, and water content |
| A260/A280 |
1.7–1.9 |
| Elution volume | 30–100 µl |
| Preparation time | 60 min/10 preps |
| Binding capacity | 50 µg |
*For human stool samples, approximately 200 mg should be used. For animal stool samples, depending on the species, a smaller amount of sample material may be required for optimal results.
Applications
- Purification of genomic DNA from bacterial and epithelial cells present in stool samples
- Typical downstream applications: PCR, real-time PCR, Southern blotting, microarray technology
Additional product information
Please see the product's Certificate of Analysis for information about storage conditions, product components, and technical specifications. Please see the Kit Components List to determine kit components. Certificates of Analysis and Kit Components Lists are located under the Documents tab.
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