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Automation systems
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Technical notes
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Next-generation sequencing
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RNA-seq
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Technotes
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Webinars
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Technical notes
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DNA-seq
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Immune Profiling
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DNA-seq
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DNA-seq protocols
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Webinars
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Stem cell research
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Gene editing in hiPS cells
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Protein research
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His-tag purification
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Protocols
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PCR
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Technical notes
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Cloning
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In-Fusion Cloning general information
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In-Fusion Cloning and competition
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Applications and technical notes
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Traditional molecular cloning
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Restriction enzyme overview
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Restriction enzyme overview
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Nucleic acid purification
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Real-time PCR
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Technical notes
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Sign up to watch the webinar
Research highlight
Refining acute leukemia genomics
The most frequent childhood cancer, acute lymphoblastic leukemia has very poor prognosis in older adolescents and adults, with survival rates below 50% for those over 40 years old. To better understand leukemic pathogenesis and its associated prognostic relevance, Dr. Ilaria Iacobucci uses single-cell sequencing to study the clonal evolution of genomic alterations in B-cell acute lymphoblastic leukemia (B-ALL) at unprecedented resolution.
Register to access a guest webinar and research spotlight about Dr. Iacobucci's comprehensive exploration of clonal heterogeneity in B-ALL and its implications for disease pathogenesis!*
If you are on a mobile device, click on the hamburger icon () on the top left of your screen, then scroll down to access the registration form.
Watch our interview with Dr. Iacobucci to learn about her inspirations, roadblocks, and most exciting single-cell discoveries.
Meet the speaker
Ilaria Iacobucci, PhD, a Principal Scientist in the Department of Pathology at St. Jude Children’s Research Hospital, Memphis (USA), earned her Ph.D. in Clinical and Experimental Hematology from the University of Bologna (Italy). She later became an Assistant Professor, investigating the mechanisms of high-risk myeloid and lymphoid leukemias in children and adults, with a focus on novel genetic alterations with diagnostic and therapeutic implications. Dr. Iacobucci serves as an Associate Editor for the journals Blood and Leukemia, and has received numerous prestigious awards for her work.
Explore materials about Dr. Iacobucci's research parsing tumor heterogeneity and identifying rare subclones in acute leukemias using high-throughput single-cell sequencing. Fill out the form to access these resources!
Guest webinar: Refining acute leukemia genomics through single-cell sequencing
In this guest webinar, Dr. Iacobucci applies single-cell whole-genome sequencing to acute leukemia samples to dissect clonal diversity at an unprecedented resolution. Her research provides insights into the dynamic process of clonal evolution and allows for a more accurate understanding of disease pathogenesis and classification.
Topics covered:
- Introduction to acute leukemias
- Limitations of bulk sequencing to study B-ALL genomic alterations
- Characterization of B-ALL subclones using the Shasta Single Cell System
Research spotlight: Identification of copy number variations (CNVs) in B-cell acute lymphoblastic leukemia utilizing the Shasta Whole-Genome Amplification (WGA) Kit
In this research spotlight, Dr. Iacobucci analyzes intra-sample heterogeneity of cord blood cells in B-ALL at scale using the Shasta Whole-Genome Amplification Kit. This exciting data offers a comprehensive resource for future B-ALL studies by complementing existing reference data sets.
Topics covered:
- Accurate detection of rare subclone harboring CNVs compared to plate-based methods
- Generation of over 85% uniquely mapped reads
- Detection of germline and somatic single nucleotide variants (SNVs) with shallow sequencing
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